3TET

Crystal Structure of NaK2K Channel Y66F Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	I 4
Unit cell lengths	67.625, 67.625, 89.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.812 - 1.900
R-factor	0.2079
Rwork	0.206
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.123
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.140	1.930
High resolution limit [Å]	1.900	2.090	1.900
Number of reflections	15737
Completeness [%]		100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	77.5% MPD, 100mM MES, 100mM KCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K