3TEK
ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.809, 103.655, 39.829 |
| Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.20738 |
| Rwork | 0.205 |
| R-free | 0.25451 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.650 | 2.051 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 19149 | |
| Completeness [%] | 91.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 11.8 % isopropanol, 0.1 M sodium citrate, 0.2 M lithium sulphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
