3TEG

Bacterial and Eukaryotic Phenylalanyl-tRNA Synthetases Catalyze Misaminoacylation of tRNAPhe with 3,4-Dihydroxy-L-Phenylalanine (L-Dopa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.93
Spacegroup name	P 21 21 21
Unit cell lengths	55.185, 90.587, 96.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.853 - 2.204
R-factor	0.1712
Rwork	0.169
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmq
RMSD bond length	0.007
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.853	2.290
High resolution limit [Å]	2.200	2.200
Number of reflections	24066
Completeness [%]	96.4	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	2 mM DTT, 100 mM Bis-Tris Propane,1.8 M Na-acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K