3TEF
Crystal Structure of the Periplasmic Catecholate-Siderophore Binding Protein VctP from Vibrio Cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.112, 73.282, 112.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.458 - 1.698 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.21320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.978 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.698 | 1.700 |
Rmerge | 0.085 | 0.597 |
Number of reflections | 30253 | |
<I/σ(I)> | 23.1 | 2.3 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 8.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M ammonium acetate, 0.1M Tris-HCl buffer, 20% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |