3TED
Crystal structure of the Chd1 DNA-binding domain in complex with a DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.670, 61.426, 76.771 |
Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.19546 |
Rwork | 0.193 |
R-free | 0.24898 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xb0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.120 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.768 | |
Number of reflections | 22675 | |
<I/σ(I)> | 37.2 | 3.2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 18.2 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 14-18% PEG 400 0.1 M BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |