3TEB
endonuclease/exonuclease/phosphatase family protein from Leptotrichia buccalis C-1013-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 206.915, 206.915, 89.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.990 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.21570 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.030 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Rmerge | 0.104 | 0.999 |
| Number of reflections | 23166 | |
| <I/σ(I)> | 36.56 | 5.17 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.8 | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.5M Magnesium formate, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
