3TE8
Crystal structure of risS periplasmic domain at low pH dimer with N-termini ordered
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.060, 56.020, 46.810 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 46.550 - 1.700 |
| R-factor | 0.1839 |
| Rwork | 0.181 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lr0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 | |
| High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
| Rmerge | 0.049 | 0.029 | 0.456 |
| Number of reflections | 22891 | 229 | 1661 |
| <I/σ(I)> | 19.09 | 35.69 | 4.53 |
| Completeness [%] | 98.1 | 82.1 | 97.4 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | BupsA.00863.i.A1 PW25353 at 2327 mg/mL against 10% PEG 1000, 10% PEG 8000, crystal tracking ID 221872c12, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
