3TE3

Coiled-coil oligomerization domain of the polycystin transient receptor potential channel PKD2L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Detector technology	CCD
Collection date	2010-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 65 2 2
Unit cell lengths	74.634, 74.634, 185.791
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.091 - 2.694
R-factor	0.255
Rwork	0.253
R-free	0.28980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.121
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.690	2.694
Number of reflections	8840
Completeness [%]		100
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1