3TDK

Crystal Structure of Human UDP-Glucose Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Detector technology	AREA DETECTOR
Collection date	2005-07-16
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	173.132, 191.177, 225.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.910 - 2.800
R-factor	0.2314
Rwork	0.230
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.462
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	145.910	49.625	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge		0.050	0.517
Rmeas		0.055	0.599
Rpim		0.021	0.292
Total number of observations		40932	89032
Number of reflections	176566
<I/σ(I)>	8	16	2.5
Completeness [%]	96.1	99.4	90.5
Redundancy	4.9	6.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	vapr diffusion	6.5	298	0.2M Ammonium sulfate, 21% PEG 8000, 0.1M Sodium cacodylate, pH 6.5, vapr diffusion, temperature 298K