3TDI
yeast Cul1WHB-Dcn1P acetylated Ubc12N complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91917 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.906, 98.421, 143.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.300 |
| R-factor | 0.20583 |
| Rwork | 0.203 |
| R-free | 0.26614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.480 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.380 | 2.300 |
| Rmerge | 0.123 | 0.370 | |
| Number of reflections | 21323 | ||
| <I/σ(I)> | 13.5 | 2.6 | |
| Completeness [%] | 96.1 | 76.6 | 65.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 295 | 20% PEG3350, 0.1M Bis-Tris Propane, 0.2M Na/K tartrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
