3TDI

yeast Cul1WHB-Dcn1P acetylated Ubc12N complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	CCD
Collection date	2010-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91917
Spacegroup name	C 2 2 21
Unit cell lengths	72.906, 98.421, 143.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.000 - 2.300
R-factor	0.20583
Rwork	0.203
R-free	0.26614
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	2.480	2.380
High resolution limit [Å]	2.300	2.380	2.300
Rmerge	0.123		0.370
Number of reflections	21323
<I/σ(I)>	13.5		2.6
Completeness [%]	96.1	76.6	65.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	295	20% PEG3350, 0.1M Bis-Tris Propane, 0.2M Na/K tartrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K