3TDC
Crystal Structure of Human Acetyl-CoA carboxylase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-02-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 113.921, 119.756, 146.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.410 |
| R-factor | 0.17163 |
| Rwork | 0.169 |
| R-free | 0.21788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uyt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.092 |
| Number of reflections | 37827 |
| <I/σ(I)> | 19.2 |
| Completeness [%] | 96.8 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 6.8% PEG MME 2000, 0.0175M Ammonium Sulfate, 0.1M MES , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
