3TD3
Crystal structure of OmpA-like domain from Acinetobacter baumannii in complex with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.108, 98.751, 98.321 |
| Unit cell angles | 90.00, 105.84, 90.00 |
Refinement procedure
| Resolution | 94.590 - 1.590 |
| R-factor | 0.18326 |
| Rwork | 0.181 |
| R-free | 0.21902 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.600 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 133854 | |
| Completeness [%] | 99.4 | 99.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M HEPES, 0.2M Ammonium sulfate, 15%-20% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
