3TD2

Crystal structures of Peptidyl-tRNA hydrolase from Mycobacterium tuberculosis - Form 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-06-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	60.310, 60.310, 87.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.23747
Rwork	0.237
R-free	0.25541
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z2i
RMSD bond length	0.009
RMSD bond angle	1.546
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.081
Number of reflections	6725
Completeness [%]	99.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch under oil	7.5	291	0.1M HEPES, 25% PEG 8000, 5% dioxane, pH 7.5, Microbatch under oil, temperature 291K