3TD0

Crystal structure of the bacterial A1408G-mutant and the protozoa cytoplasmic ribosomal decoding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.520, 46.730, 55.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.700 - 1.600
Rwork	0.220
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.800
Data reduction software	CrystalClear
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.700	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.049	0.235
Number of reflections	16037
Completeness [%]	99.4	100
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Cacodylate, Spermine tetrahydrochloride, Magnesium chloride, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Cacodylate, Spermine tetrahydrochloride, Magnesium chloride, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K