3TCI
Crystal structure of the decameric sequence d(CGGGCGCCCG) as Z type duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 17.767, 17.767, 42.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.387 - 2.421 |
| R-factor | 0.1838 |
| Rwork | 0.183 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.603 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.061 | 0.271 |
| Number of reflections | 579 | |
| <I/σ(I)> | 4.4 | 1.7 |
| Completeness [%] | 98.8 | 96.9 |
| Redundancy | 3.41 | 3.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1mM DNA, 2M MgCl2, 1mM spermine, 50% MPD , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
