3TCF
Crystal structure of E. coli OppA complexed with endogenous ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.619, 202.599, 208.947 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 48.370 - 2.000 |
| R-factor | 0.2306 |
| Rwork | 0.229 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.332 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.950 | 2.120 |
| High resolution limit [Å] | 2.000 | 4.220 | 2.000 |
| Rmerge | 0.079 | 0.031 | 0.554 |
| Number of reflections | 345441 | 23801 | 53085 |
| <I/σ(I)> | 12.41 | 35.27 | 2.26 |
| Completeness [%] | 98.9 | 99.9 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 8000, glycerol, pH 6.0, vapor diffusion, hanging drop, temperature 293K |
