3TBY
CRYSTAL STRUCTURE OF THE MURINE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX H-2DB IN COMPLEX WITH THE LCMV-DERIVED GP33 ALTERED PEPTIDE ligand (V3P, Y4F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93400 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.820, 124.270, 99.650 |
Unit cell angles | 90.00, 103.29, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.28604 |
Rwork | 0.284 |
R-free | 0.31473 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.192 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.112 | 0.631 |
Number of reflections | 75693 | |
<I/σ(I)> | 10.7 | 1.9 |
Completeness [%] | 98.1 | 99.2 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Crystals were obtained in 1.6-1.8 M ammonium sulfate, 0.1 M Tris HCl pH 7.0-9.0 screening conditions. 4 ul of a 5mg/ml protein solution were mixed in a 4:2 ratio with the crystallization reservoir , VAPOR DIFFUSION, HANGING DROP, temperature 293K |