3TBN
Crystal structure of a miner2 homolog: a type 6 CDGSH iron-sulfur protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 63.498, 63.498, 49.068 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.057 - 1.150 |
| R-factor | 0.112 |
| Rwork | 0.111 |
| R-free | 0.12510 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.106 | 0.494 |
| Number of reflections | 39768 | |
| <I/σ(I)> | 33.5 | 4.9 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 9.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 4.0M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
