3TBL

Structure of Mono-ubiquitinated PCNA: Implications for DNA Polymerase Switching and Okazaki Fragment Maturation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	161.055, 161.055, 97.357
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.599 - 2.903
R-factor	0.2252
Rwork	0.222
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	114.000
High resolution limit [Å]	2.900
Number of reflections	28695
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.9 to 1.3 M sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K