3TBD
Crystal Structure of domain VI and LE1 of human Netrin-G2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.071 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.134, 63.134, 190.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.270 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 | 1.860 |
| High resolution limit [Å] | 1.800 | 2.270 | 1.800 |
| Number of reflections | 40558 | ||
| Completeness [%] | 100 | 74.8 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 20% PEG 3350, 0.2M Calcium acetate, 0.1M Sodium cacodylate pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
