3TB6
Structure of the effector-binding domain of arabinose repressor AraR from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.068, 106.333, 111.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 2.210 |
| R-factor | 0.18808 |
| Rwork | 0.186 |
| R-free | 0.23099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jft |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.570 | 0.280 |
| Number of reflections | 33024 | |
| <I/σ(I)> | 35.9 | 4.9 |
| Completeness [%] | 86.8 | 59.6 |
| Redundancy | 66 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | arabinose 50mM at 16.8mg/ml, 70mM HEPES pH7.5, 7% PEG8000, 6% ethylene glycol, 20% glycerol, 10mM spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
