3TB5
Crystal Structure of the Enterococcus faecalis Methionine aminopeptidase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99190 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.740, 132.130, 85.300 |
| Unit cell angles | 90.00, 133.36, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.300 |
| R-factor | 0.19995 |
| Rwork | 0.197 |
| R-free | 0.25484 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c21 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.110 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.220 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.052 |
| Number of reflections | 43107 |
| Completeness [%] | 99.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 14-20% PEG 3400, 0.14M (NH4)2H-citrate, 8% glycerol, pH 5.4-6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
