3TAV
Crystal structure of a Methionine Aminopeptidase from Mycobacterium abscessus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979175 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 119.640, 119.640, 96.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
| Rmerge | 0.071 | 0.045 | 0.385 |
| Number of reflections | 43354 | 400 | 3207 |
| <I/σ(I)> | 13.94 | 20.8 | 3.3 |
| Completeness [%] | 99.4 | 71.8 | 99.9 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | MyabA.01548.b.A1 PS01037 at 56.7 mg/mL with full 21 aa tag against JCSG + f9, 2.4 M Na malonate with 25% ethylene glycol as cryo-protectant, crystal tracking ID 222124f9, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
