3TAS

Small laccase from Streptomyces viridosporus T7A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-04
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	I 2 2 2
Unit cell lengths	102.300, 157.180, 162.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.692 - 2.300
R-factor	0.149
Rwork	0.147
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cg8
RMSD bond length	0.008
RMSD bond angle	1.269
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_764)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.221	0.053	0.842
Number of reflections	58241	619	4267
<I/σ(I)>	10.51	32.85	2.02
Completeness [%]	99.8	84.1	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	282	Protein concentration was 20 mg/mL, containing 50 mM Hepes-K+ (pH 8.0), and 100 mM KCl; the precipitant contained 2.0 M Li2SO4, 5% polyethylene glycol 400, 100 mM MgSO4, 100 mM acetate, VAPOR DIFFUSION, HANGING DROP, temperature 282K