3TAQ
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to Duplex DNA with Cytosine-Adenine Mismatch at (n-4) Position
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97121 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.580, 93.600, 106.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.784 - 1.650 |
R-factor | 0.1678 |
Rwork | 0.167 |
R-free | 0.19000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1l5u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.243 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.750 |
High resolution limit [Å] | 1.650 | 4.910 | 1.650 |
Rmerge | 0.046 | 0.016 | 0.470 |
Number of reflections | 101635 | ||
<I/σ(I)> | 21.17 | 72.97 | 3.36 |
Completeness [%] | 97.4 | 89.4 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 45-50% saturated ammonium sulfate, 2.5% MPD, 10 mM magnesium sulfate, 100 mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |