3TA2
A. fulgidus GlnK3, MgATP/2-OG complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 79.220, 79.220, 223.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 1.900 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o8w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.144 | |
| Number of reflections | 33612 | |
| <I/σ(I)> | 18 | 4.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% MPD, 0.2 M sodium chloride, 0.1 M sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
