3T98
Molecular Architecture of the Transport Channel of the Nuclear Pore Complex: Nup54/Nup58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 54.960, 54.960, 190.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.253 |
| Rwork | 0.250 |
| R-free | 0.27200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11076 | |
| <I/σ(I)> | 23 | 4.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | Protein concentration 14-17 mg/ml Drop size 2.4 ul Reservoir: 0.1 M Sodium Acetate pH 3.8-4.1 and 0.08-0.1 M CaCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
