3T97
Molecular Architecture of the Transport Channel of the Nuclear Pore Complex: Nup62/Nup54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.010, 62.350, 121.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.2305 |
| Rwork | 0.235 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 4819 | |
| <I/σ(I)> | 41.4 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 18-22% PEG 4000,0.1M Tris-acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
