3T8Y

Crystal structure of the response regulator domain of Thermotoga maritima CheB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.949, 0.950, 0.918
Spacegroup name	P 21 21 21
Unit cell lengths	53.715, 53.679, 131.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
Rwork	0.229
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.327
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge		0.356
Number of reflections	54997
<I/σ(I)>		34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	15-20% t-Butanol, 100mM MES, 10% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K