3T8O

Rhodopsin kinase (GRK1) L166K mutant at 2.5A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9999
Spacegroup name	I 2 2 2
Unit cell lengths	55.541, 149.808, 190.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.500
R-factor	0.2088
Rwork	0.207
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.540
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.086	0.036	0.792
Number of reflections	27804
<I/σ(I)>	8
Completeness [%]	99.5	99.6	99
Redundancy	8.2	7.6	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	293	12% PEG 6000, 100 mM Na-citrate pH 4.3 and 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K