3T8B
Crystal structure of Mycobacterium tuberculosis MenB with altered hexameric assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.233, 132.233, 71.139 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.977 - 1.649 |
| R-factor | 0.1434 |
| Rwork | 0.141 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q51 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.830 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.649 | 4.480 | 1.650 |
| Rmerge | 0.083 | 0.052 | 0.681 |
| Number of reflections | 55893 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 5.7 | 5.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 19% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
