3T7Z
Structure of Methanocaldococcus jannaschii Nop N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0, 0.97924 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 45.695, 86.511, 129.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.958 - 1.700 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.193 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.073 | 0.376 |
| Number of reflections | 12790 | |
| <I/σ(I)> | 38.4 | 2.95 |
| Completeness [%] | 89.0 | 38.3 |
| Redundancy | 5.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 18% PEG 8000, 0.1M Sodium cacodylate, 0.2M Zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
