3T7I

Crystal structure of Se-Met Rtt107p (residues 820-1070)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9805, 0.9807, 0.9567
Spacegroup name	P 21 21 21
Unit cell lengths	59.236, 74.636, 129.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.986 - 2.300
R-factor	0.2055
Rwork	0.203
R-free	0.24810
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.106	0.063	0.313
Number of reflections	26232
<I/σ(I)>	11.5
Completeness [%]	99.8	97.8	100
Redundancy	12.2	11	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	283	17.5%(w/v) MPEG2000, 0.1M HEPES, 0.2M NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K