3T7F

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2009-12-05
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	56.856, 74.677, 76.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.880 - 1.890
R-factor	0.177
Rwork	0.175
R-free	0.19700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.890
Number of reflections	26234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4	277	9.2% isopropanol, 0.1M potassium thiocyanate, 0.1M citrate buffer pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K