3T7D
Vall from streptomyces hygroscopicus in complex with trehalose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.661, 45.820, 115.030 |
| Unit cell angles | 90.00, 107.36, 90.00 |
Refinement procedure
| Resolution | 39.780 - 1.700 |
| R-factor | 0.16504 |
| Rwork | 0.162 |
| R-free | 0.21369 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.432 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.780 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 82832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 30% PEG4000, 200mM MgCl2, 100mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
