3T7B
Crystal Structure of N-acetyl-L-glutamate kinase from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97903 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 126.456, 134.688, 98.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.1799 |
Rwork | 0.178 |
R-free | 0.21340 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.540 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.084 | 0.069 | 0.656 |
Number of reflections | 29446 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 9.3 | 8.3 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | PEG3350 20%, NaTartrate 0.2M, 10mM ADP, 5mM Glutamic Acid, pH 7, VAPOR DIFFUSION, HANGING DROP |