3T79
Ndc10: a platform for inner kinetochore assembly in budding yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.060, 99.651, 125.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.645 - 3.611 |
| R-factor | 0.2885 |
| Rwork | 0.286 |
| R-free | 0.33090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sqi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.223 | 0.912 |
| Number of reflections | 13821 | |
| <I/σ(I)> | 1.4 | 1.4 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG 10,000, 0.1 M Tris-HCl, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
