3T6V
Crystal Structure of Laccase from Steccherinum ochraceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-28 |
| Detector | MARIP, 345 MM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.535, 140.662, 174.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qt6 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.170 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.330 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.367 |
| Number of reflections | 120568 | |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 98.6 | 95.8 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 296 | 1.9 M ammonium sulphate, 0.01 M zinc chloride, pH 9.0, vapor diffusion, temperature 296K |
