3T69
Crystal structure of a putative 2-dehydro-3-deoxygalactonokinase protein from Sinorhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.398, 109.741, 121.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.460 - 2.550 |
| R-factor | 0.22032 |
| Rwork | 0.217 |
| R-free | 0.27739 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.600 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 27367 | |
| <I/σ(I)> | 7.6 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M BIS-TRIS pH5.5, 2M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
