3T5M
Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 |
| Unit cell lengths | 118.389, 118.389, 55.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.714 - 1.749 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.714 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 77550 | |
| <I/σ(I)> | 23 | 3 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M potassium chloride, 20% Peg 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
