3T5D
Crystal structure of Septin 7 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-08-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 61 |
| Unit cell lengths | 82.363, 82.363, 210.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.250 - 3.300 |
| R-factor | 0.28696 |
| Rwork | 0.285 |
| R-free | 0.31826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.912 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.250 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.392 | |
| Number of reflections | 12079 | |
| <I/σ(I)> | 3.85 | |
| Completeness [%] | 99.1 | 98.9 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.2M sodium chlorid, 1.1M ammonium sulfate, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
