3T5A
Crystal structure of N-terminal domain of FAAL28 G330W mutant from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-11-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.207, 60.317, 137.773 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.050 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e53 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.098 | 0.486 |
Number of reflections | 25902 | |
<I/σ(I)> | 14.63 | 2.03 |
Completeness [%] | 92.9 | 94 |
Redundancy | 4.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 18% PEG 3350, 0.1M Sodium MES, 5% ethylene glycol, 0.15M Lithium sulphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |