3T51
Crystal structures of the pre-extrusion and extrusion states of the CusBA adaptor-transporter complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 159.639, 159.639, 679.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.900 |
| R-factor | 0.2619 |
| Rwork | 0.259 |
| R-free | 0.32470 |
| Structure solution method | MR_SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 28809 | |
| Completeness [%] | 93.8 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 10% PEG3350, 0.4M NH4SO4, 10% Glycerol, pH 7.0, vapor diffusion, temperature 298K, VAPOR DIFFUSION |
