3T4Z
Crystal Structure of NaK2K Channel Y55W Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9790983 |
| Spacegroup name | I 4 |
| Unit cell lengths | 68.388, 68.388, 86.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.835 - 1.901 |
| R-factor | 0.2265 |
| Rwork | 0.226 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 | 1.930 |
| High resolution limit [Å] | 1.900 | 2.090 | 1.900 |
| Number of reflections | 14653 | ||
| Completeness [%] | 89.4 | 74.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 65% MPD, 100mM Glycine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
