3T4R
Lettuce Necrotic Yellow Virus Phosphoprotein C-Terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.974 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 43.280, 43.280, 89.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.600 - 2.000 |
| R-factor | 0.22922 |
| Rwork | 0.226 |
| R-free | 0.29351 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.064 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.455 |
| Number of reflections | 6220 | |
| <I/σ(I)> | 20.62 | 2.88 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 10.56 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50 mM Na Cacodylate, 40 mM Magnesium Acetate, 10% MPD, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
