3T4Q

Arabidopsis histidine kinase 4 sensor domain in complex with trans-zeatin riboside (hydrolysed)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-12-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.900, 59.900, 297.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.360 - 2.300
R-factor	0.1814
Rwork	0.178
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t4j
RMSD bond length	0.002
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.360	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	28135
<I/σ(I)>	14.9	5.27
Completeness [%]	97.9	95.9
Redundancy	3.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	16% PEG 3,350, 0.2 M Na malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K