3T4B
Crystal Structure of the HCV IRES pseudoknot domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159, 1.4855, 1.4370 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.390, 113.390, 88.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.089 - 3.550 |
| R-factor | 0.2227 |
| Rwork | 0.221 |
| R-free | 0.25420 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.769 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.640 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 7348 | |
| <I/σ(I)> | 3.02 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | RNA was mixed in a 1:1 ratio with well solution (0.5-0.6 M Li2SO4, 30-37 mM MgCl2, 1-2 mM spermine, 50 mM MES, pH 6.5) with an additional 10 mM NiCl2 added to the drop before equilibration, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
