3T3I
Glycogen Phosphorylase b in complex with GlcCF3U
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-05-31 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.759, 128.759, 116.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.780 - 2.650 |
| R-factor | 0.16274 |
| Rwork | 0.159 |
| R-free | 0.23511 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gpn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.780 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.099 | 0.429 |
| Number of reflections | 27634 | |
| <I/σ(I)> | 12.3 | 1.9 |
| Completeness [%] | 95.6 | 87.3 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | T state crystals soaked with 2 mM solution of the inhibitor in the crystallization media for 2hrs, pH 6.7, SMALL TUBES, temperature 289K |
