3T3G
Glycogen Phosphorylase b in complex with GlcBrU
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2011-04-06 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5419 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.776, 128.776, 116.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.670 - 2.400 |
R-factor | 0.18164 |
Rwork | 0.179 |
R-free | 0.23486 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2gpn |
RMSD bond length | 0.018 |
RMSD bond angle | 1.777 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.870 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.108 | 0.513 |
Number of reflections | 36619 | |
<I/σ(I)> | 7.2 | 1.3 |
Completeness [%] | 95.0 | 94.5 |
Redundancy | 2.3 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | T state crystals soaked with 2 mM solution of the inhibitor in the crystallization media for 2hrs, pH 6.7, SMALL TUBES, temperature 289K |