3T38
Corynebacterium glutamicum thioredoxin-dependent arsenate reductase Cg_ArsC1'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 77.36 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.800, 75.500, 58.100 |
| Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
| Resolution | 24.380 - 2.200 |
| R-factor | 0.17987 |
| Rwork | 0.177 |
| R-free | 0.23103 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.795 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.370 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 35383 | |
| Completeness [%] | 75.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 26% PEG4000, 0.1M Tris, 0.1M NaClO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
